Cortactin Antibody

ProSci
Product Code: PSI-45-044
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-044-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-044 (0.01ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-044 (0.01ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
EMS1, CORTACTIN, ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate), Cttn, oncogene EMS1, CTTN, FLJ34459, 1110020L01Rik
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 80kDa band observed in Human, Mouse and Rat Brain lysates and in lysates of cell line HEK293 (calculated MW of 61.6kDa according to Human NP_005222.2 and of 61.2kDa according to Mouse NP_031829.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Paradis et al, J Cell Sci. 2008 Jun 15;121(Pt 12):1965-72; PMID 18495841). Recommended concentration: 0.01-0.03ug/ml.
Background References:
  • Schuuring E, Verhoeven E, Mooi WJ, Michalides RJ. Identification and cloning of two overexpressed genes, U21B31/PRAD1 and EMS1, within the amplified chromosome 11q13 region in human carcinomas. Oncogene 1992 Feb;7(2):355-61
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: WKASAGHAVSIA-C
NCBI Gene ID #:
2017
NCBI Official Name:
cortactin
NCBI Official Symbol:
CTTN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005222.2, NP_612632.1
Protein GI Number:
20357552
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_005222.2; NP_612632.1).
Swissprot #:
Q14247