D54 Antibody

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ProSci
Product Code: PSI-45-455
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-455-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-455 (0.1ug/ml) staining of Human Breast Cancer lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-455 (3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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45-455 (0.1ug/ml) staining of Human Breast Cancer lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-455 (3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Documents

Further Information

Additional Names:
TPD52L2, D54, tumor protein D52-like 2 , DKFZp686A1765, hD54, HCCR-binding protein 2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 28kDa band observed in Human Breast Cancer and Human Brain lysates (calculated MW of 24.9kDa according to NP_955392.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour. See also our D52 and D53 products.Immunohistochemistry:Paraffin embedded Human Spleen, Tonsil and Brain (Cortex). Recommended concentration: 3.75ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Background References:
  • Reynolds JL, Mahajan SD, Sykes D, Nair MP. Heroin-Induces Differential Protein Expression by Normal Human Astrocytes (NHA). Am J Infect Dis. 2006;2(2):49-57.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-SGDKPLSDPAP
NCBI Gene ID #:
7165
NCBI Official Name:
tumor protein D52-like 2
NCBI Official Symbol:
TPD52L2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_955392.1, NP_955393.1, NP_955394.1, NP_955395.1, NP_003279.2, NP_955391.1
Protein GI Number:
40805864
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise all reported isoforms (NP_955392.1; NP_955393.1; NP_955394.1; NP_955395.1; NP_003279.2; NP_955391.1).
Swissprot #:
O43399