DSCAM Antibody
| Code | Size | Price |
|---|
| PSI-45-499-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
DSCAM, Down syndrome cell adhesion molecule , CHD2-42, CHD2-52 , human CHD2-52 down syndrome cell adhesion molecule
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 200-220kDa band observed in HepG2 cell lysates (calculated MW of 174kDa according to NP_996770.1 ). The observed molecular weight corresponds to different antibodies from other commercial sources. Recommended concentration: 2-3ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.
Background References:
- Li W, Guan KL. The Down syndrome cell adhesion molecule (DSCAM) interacts with and activates Pak. J Biol Chem. 2004 Jul 30;279(31):32824-31. Epub 2004 May 28.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-DSWDSAQRTKDVSPQ
NCBI Gene ID #:
1826
NCBI Official Name:
Down syndrome cell adhesion molecule
NCBI Official Symbol:
DSCAM
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001380.2, NP_001258463.1
Protein GI Number:
20127422
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise both reported isoforms (NP_001380.2 and NP_996770.1).
Swissprot #:
O60469