IGF-BP5 Antibody
| Code | Size | Price |
|---|
| PSI-38-289-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repea
Documents
Further Information
Additional Names:
IBP5, IBP5, Insulin-like growth factor-binding protein 5IBP-5
Application Note:
ELISA:
Sandwich:
To detect hIGF-BP5 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Polyclonal Biotinylated Anti-Human IGF-BP5 (38-154) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP5.
Western Blot:
: To detect hIGF-BP5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Immunohistochemistry:
This antibody stained U-2 OS and U-251 MG cells. The primary antibody was incubated at 2.0 mg/mL overnight at 4˚C followed by a fluorescent labeled secondary antibody.
Sandwich:
To detect hIGF-BP5 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Polyclonal Biotinylated Anti-Human IGF-BP5 (38-154) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP5.
Western Blot:
: To detect hIGF-BP5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Immunohistochemistry:
This antibody stained U-2 OS and U-251 MG cells. The primary antibody was incubated at 2.0 mg/mL overnight at 4˚C followed by a fluorescent labeled secondary antibody.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
E.coli derived Recombinant Human IGF-BP5
NCBI Gene ID #:
3488
NCBI Official Name:
insulin-like growth factor binding protein 5
NCBI Official Symbol:
IGFBP5
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P24593
Protein GI Number:
124069
Purification:
IGF-BP5 specific antibody was purified by affinity chromatography employing immobilized hIGF-BP5 matrix.
Research Area:
Apoptosis,Cancer,Antibody Pairs
Swissprot #:
P24593
User NOte:
Centrifuge vial prior to opening.