IL-10 Monoclonal Antibody
| Code | Size | Price |
|---|
| PSI-38-301-0.5mg | 0.5mg | £385.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repea
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Further Information
Additional Names:
Interleukin 10Interleukin-10, Cytokine synthesis inhibitory factor, IL-10, CSIF, TGIF, GVHDS, IL10A
Application Note:
ELISA:
Sandwich:
In a sandwich ELISA (assuming 100μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 1600 pg/mL of recombinant murine IL-10 when used with ProSci?s Polyclonal biotinylated antigen affinity purified anti-murine IL-10 as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western Blot:
To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-10 (0.5 ng/mL), a concentration of 3.0-5.0 μg/mL of this antibody is required.
Sandwich:
In a sandwich ELISA (assuming 100μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 1600 pg/mL of recombinant murine IL-10 when used with ProSci?s Polyclonal biotinylated antigen affinity purified anti-murine IL-10 as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western Blot:
To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-10 (0.5 ng/mL), a concentration of 3.0-5.0 μg/mL of this antibody is required.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
E.coli derived Recombinant Murine IL-10
NCBI Gene ID #:
3586
NCBI Official Name:
interleukin 10
NCBI Official Symbol:
IL10
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
NP_000563.1
Protein GI Number:
10835141
Purification:
This IgG2A antibody was purified from cell culture by Protein G affinity chromatography.
Research Area:
Infectious Disease,Neuroscience,Cancer,Stem Cell,Chemokines & Cytokines, Functional Assays
Swissprot #:
P22301
User NOte:
Centrifuge vial prior to opening.