IL-2 Monoclonal Antibody
| Code | Size | Price |
|---|
| PSI-38-299-0.5mg | 0.5mg | £385.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repea
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Further Information
Additional Names:
Interleukin 2Interleukin-2, T-cell growth factor, IL-2, TCGF, lymphokine
Application Note:
ELISA:
Sandwich:
In a sandwich ELISA (assuming 100μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 32 pg/mL of recombinant murine IL-2 when used with ProSci?s Polyclonal biotinylated antigen affinity purified anti-murine IL-2 (XP-5183Bt) as the detection antibody at a concentration of at least 1.0-2.0 μg/mL.
Western Blot:
To detect mIL-2 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-2 is 1.0-2.0 ng/lane, under reducing conditions and 0.2-0.4 ng/lane, under non-reducing conditions.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-2 (1.0 ng/mL), a concentration of 10.0-14.0 μg/mL of this antibody is required.
Sandwich:
In a sandwich ELISA (assuming 100μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 32 pg/mL of recombinant murine IL-2 when used with ProSci?s Polyclonal biotinylated antigen affinity purified anti-murine IL-2 (XP-5183Bt) as the detection antibody at a concentration of at least 1.0-2.0 μg/mL.
Western Blot:
To detect mIL-2 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-2 is 1.0-2.0 ng/lane, under reducing conditions and 0.2-0.4 ng/lane, under non-reducing conditions.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-2 (1.0 ng/mL), a concentration of 10.0-14.0 μg/mL of this antibody is required.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
E.coli derived Recombinant Murine IL-2
NCBI Gene ID #:
3558
NCBI Official Name:
interleukin 2
NCBI Official Symbol:
IL2
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P60568
Protein GI Number:
45593462
Purification:
This IgG2Ak antibody was purified from cell culture by Protein A affinity chromatography.
Research Area:
Infectious Disease,Neuroscience,Cancer,Stem Cell,Chemokines & Cytokines, Functional Assays
Swissprot #:
P60568
User NOte:
Centrifuge vial prior to opening.