FMR1 Antibody

ProSci
Product Code: PSI-46-842
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-842-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-842 (0.1ug/ml) staining of KELLY cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-842 (0.1ug/ml) staining of KELLY cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
FMR1, fragile X mental retardation 1 , FMRP, FRAXA, MGC87458
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 80kDa band observed in lysates of cell line KELLY (calculated MW of 71.2kDa according to NP_002015.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Sittler et al, Hum Mol Genet. 1996 Jan;5(1):95-102; PMID: 8789445). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Placenta. Recommended concentration: 2.5ug/ml.
Background References:
  • Hanson JE, Blank M, Valenzuela RA, Garner CC, Madison DV. The functional nature of synaptic circuitry is altered in area CA3 of the hippocampus in a mouse model of Down's syndrome. J Physiol. 2007 Feb 15;579(Pt 1):53-67. Epub 2006 Dec 7.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-RTGKDRNQKKEKPD
NCBI Gene ID #:
2332
NCBI Official Name:
fragile X mental retardation 1
NCBI Official Symbol:
FMR1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002015.1
Protein GI Number:
4503765
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience
SPECIFICITY:
.
Swissprot #:
Q06787