FOXG1 Antibody

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ProSci
Product Code: PSI-45-053
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-053-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-053 (0.3ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-053 (0.3ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Documents

Further Information

Additional Names:
forkhead box G1, BF1, BF2, FHKL3, FKH2, FKHL1, FKHL2, FKHL3, FKHL4, FOXG1A, FOXG1B, FOXG1C, HBF-1, HBF-2, HBF-3, HBF-G2, HBF2, HFK1, HFK2, HFK3, KHL2, QIN, OTTHUMP00000178764, brain factor 2, forkhead box G1A, forkhead box G1B, forkhead box G1C, forkhead, drosophila, homolog-like 2, forkhead-like 1, forkhead-like 2, forkhead-like 3, forkhead-like 4, oncogene QIN
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 48 kDa band observed in Human Brain lysates (calculated MW of 52.4kDa according to NP_005240.3 (FOXG1B) and 50.5kDa according to CAA55038.1 (FOXG1A)). Recommended concentration: 0.3-1.0ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 70kDa. This band was successfully blocked by incubation with the immunising peptide. Preliminary testing was unsuccessful on Mouse and Rat Brain for this particular batch.
Background References:
  • Wiese S, Murphy DB, Schlung A, Burfeind P, Schmundt D, Schnulle V, Mattei MG, Thies U. The genes for human brain factor 1 and 2, members of the fork head gene family, are clustered on chromosome 14q. Biochim Biophys Acta. 1995 Jun 9;1262(2-3):105-12.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-HQNQGSSSNPLIH
NCBI Gene ID #:
2290
NCBI Official Name:
forkhead box G1
NCBI Official Symbol:
FOXG1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005240.3
Protein GI Number:
967048
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
.
Swissprot #:
P55316