IDH3A Antibody

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ProSci
Product Code: PSI-42-381
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-381-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-381 (0.1ug/ml) staining of Human Lymph Nodes lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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42-381 (3.75ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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42-381 (0.1ug/ml) staining of Human Lymph Nodes lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-381 (3.75ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Documents

Further Information

Additional Names:
IDH3A, isocitrate dehydrogenase 3 (NAD+) alpha, H-IDH alpha, NAD(+)-specific ICDH subunit alpha, NAD(H)-specific isocitrate dehydrogenase alpha subunit, NAD+-specific ICDH, isocitrate dehydrogenase (NAD+) alpha chain, isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial, isocitric dehydrogenase subunit alpha
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 37kDa band observed in lysates of Human Lymph Nodes, and of cell line DAUDI, and 38kDa in Human, Mouse and Rat Spleen lysates and in Pig Spleen and Skeletal Muscle lysates (calculated MW of 39.6kDa according to NP_005521.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Thymus. Recommended concentration: 3.75ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: DFTEEICRRVKDLD
NCBI Gene ID #:
3419
NCBI Official Name:
isocitrate dehydrogenase 3 (NAD+) alpha
NCBI Official Symbol:
IDH3A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005521.1
Protein GI Number:
5031777
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
.
Swissprot #:
P50213