IRF5 Antibody
| Code | Size | Price |
|---|
| PSI-45-075-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- ChIP
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunoprecipitation (IP)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
IRF5, interferon regulatory factor 5, SLEB10
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in Human Spleen and Peripheral Blood Lymphocytes lysates.The calculated molecular size is 57.8kDa according to NP_001092099.1. Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:This antibody showed nuclear staining in Human Cervix, as confirmed by customer (data not shown). This data is from a previous batch, not on sale.Chromatin immunoprecipitation: This antibody has been successfully used in human monocyte-derived dendritic cells.This data is from a previous batch, not on sale.
Background References:
- Barnes BJ, Moore PA, Pitha PM. Virus-specific activation of a novel interferon regulatory factor, IRF-5, results in the induction of distinct interferon alpha genes. J Biol Chem. 2001 Jun 29;276(26):23382-90.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Cow
Immunogen:
The immunogen for this antibody is: C-QGPWPMHPAGMQ
NCBI Gene ID #:
3663
NCBI Official Name:
interferon regulatory factor 5
NCBI Official Symbol:
IRF5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002191.1, NP_116032.1, NP_001092097.1, NP_001092099.1
Protein GI Number:
4504727
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Immunology ,Apoptosis
SPECIFICITY:
This antibody is expected to recognize all four reported isoforms (NP_002191.1; NP_116032.1; NP_001092097.1; NP_001092099.1). Reported variants NP_001092100.1, NP_001092101.1, NP_001092098.1, NP_001092097.1 represent identical protein: Reported variants NP_116032.1, NP_001092100.1 represent identical protein:
Swissprot #:
Q13568