LCP2 Antibody

ProSci
Product Code: PSI-45-081
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-081-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-081 (0.3ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

45-081 (0.3ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
LCP2, SLP76, SLP-76, lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa), 76 kDa tyrosine phosphoprotein, SH2 domain-containing leukocyte protein of 76kD, lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kD), lymphocyte cytosolic protein 2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 80kDa band observed in lysates of cell line Jurkat (calculated MW of 60.2kDa according to NP_005556.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Jackman et al, J Biol Chem. 1995 Mar 31;270(13):7029-32. PMID: 7706237). Recommended concentration: 0.3-1ug/ml.
Background References:
  • Jackman JK, Motto DG, Sun Q, Tanemoto M, Turck CW, Peltz GA, Koretzky GA, Findell PR. Molecular cloning of SLP-76, a 76- kDa tyrosine phosphoprotein associated with Grb2 in T cells. J Biol Chem. 1995 Mar 31;270(13):7029-32.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: ALRNVPFRSEV-C
NCBI Gene ID #:
3937
NCBI Official Name:
lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa)
NCBI Official Symbol:
LCP2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005556.1
Protein GI Number:
5031855
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
.
Swissprot #:
Q13094