Leptin Monoclonal Antibody
| Code | Size | Price |
|---|
| PSI-38-235-0.5mg | 0.5mg | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
Leptin lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4
Documents
Further Information
Additional Names:
OB, OBS, Leptin, Obese proteinOB, LEPD
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-2 (1.0 ng/mL), a concentration of 10.0-14.0 μg/mL of this antibody is required. ELISA:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 32 pg/mL of recombinant murine IL-2 when used with our biotinylated antigen affinity purified anti-murine IL-2 (XP-5183Bt) as the detection antibody at a concentration of at least 1.0-2.0 μg/mL.
Western Blot:
To detect mIL-2 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-2 is 1.0-2.0 ng/lane, under reducing conditions and 0.2-0.4 ng/lane, under non-reducing conditions.
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-2 (1.0 ng/mL), a concentration of 10.0-14.0 μg/mL of this antibody is required. ELISA:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 32 pg/mL of recombinant murine IL-2 when used with our biotinylated antigen affinity purified anti-murine IL-2 (XP-5183Bt) as the detection antibody at a concentration of at least 1.0-2.0 μg/mL.
Western Blot:
To detect mIL-2 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-2 is 1.0-2.0 ng/lane, under reducing conditions and 0.2-0.4 ng/lane, under non-reducing conditions.
Background:
Leptin plays a critical role in the regulation of body weight by inhibiting food intake and stimulating energy expenditure. Defects in Leptin production cause severe hereditary obesity in rodents and humans. In addition to its effects on body weight, leptin has a variety of other functions, including the regulation of hematopoiesis, angiogenesis, wound healing, and the immune and inflammatory response. The Leptin gene is the human homolog of the gene (ob) mutant in the mouse 'obese' phenotype.
Was product number XP-5712-M
Was product number XP-5712-M
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Leptin antibody is produced in BALB/c mice using highly pure (>98%) recombinant human Leptin as the immunizing antigen.
NCBI Gene ID #:
3952
NCBI Official Name:
leptin
NCBI Official Symbol:
LEP
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P41159
Protein GI Number:
730218
Purification:
This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.
Research Area:
Signal Transduction,Growth Factor,Obesity,Chemokines & Cytokines, Functional Assays
Swissprot #:
P41159
User NOte:
Centrifuge vial prior to opening.