MEST Antibody

ProSci
Product Code: PSI-42-158
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-158-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 1
42-158 (0.2ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

42-158 (0.2ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
MEST, mesoderm specific transcript homolog (mouse), DKFZp686L18234, MGC111102, MGC8703, PEG1, mesoderm specific transcript, paternally expressed gene 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 37kDa band observed in Mouse and Rat Testis lysates (calculated MW of 37.6kDa according to Human NP_803490.1 and 38.8kDa according to Rat NP_001009617.1). Recommended concentration: 0.2-0.5ug/ml.
Background References:
  • Li T, Vu TH, Lee KO, Yang Y, Nguyen CV, Bui HQ, Zeng ZL, Nguyen BT, Hu JF, Murphy SK, Jirtle RL, Hoffman AR, An imprinted PEG1/MEST antisense expressed predominantly in human testis and in mature spermatozoa. J. Biol. Chem. 2002 Apr 277 (16): 13518-27.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-QELLYRYKQNRSGR
NCBI Gene ID #:
4232
NCBI Official Name:
mesoderm specific transcript
NCBI Official Symbol:
MEST
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002393.2, NP_803490.1
Protein GI Number:
29294639
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_002393.2; NP_803490.1). Reported variants NP_803490.1 and NP_803491.1 represent identical protein.
Swissprot #:
Q5EB52