MIP-3 beta Monoclonal Antibody

ProSci
Product Code: PSI-38-237
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-38-237-0.5mg0.5mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
MIP-3-beta lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at

Images

1 / 1
This antibody stained PBMC.  The primary antibody was incubated at 5.0 mg/ml for one hour at room temperature followed by a fluorescent labeled secondary antibody.  Optimal concentrations and conditions may vary.  Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia.

This antibody stained PBMC.  The primary antibody was incubated at 5.0 mg/ml for one hour at room temperature followed by a fluorescent labeled secondary antibody.  Optimal concentrations and conditions may vary.  Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia.

Further Information

Additional Names:
Macrophage Inflammatory Protein 3 beta, SCYA20, Small inducible cytokine A20, LARC, Liver and activation regulated chemokine, exodus-1, MIP3, MPIF1, SCYA23, C-C motif chemokine 23, CK-beta-8, CKB-8, CKb8, Ckb-8, MIP-3, MPIF-1, Ckb-8-1, hmrp-2a, CK-BETA-8
Application Note:
ELISA:

In a sandwich ELISA (assuming 100 μL/well), a concentration of 14.0-16.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant rat IL-2 when used with our biotinylated antigen affinity purified anti-rat IL-2 (38-205) as the detection antibody at a concentration of at least 2.0-4.0 μg/mL.

Western Blot:

To detect rat IL-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant rat IL-2 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
Background:
Was product number XP-5714-M
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
MIP-3-beta antibody is produced in BALB/c mice using highly pure (>98%) recombinant human MIP-3beta as the immunizing antigen.
NCBI Gene ID #:
6368
NCBI Official Name:
chemokine (C-C motif) ligand 23
NCBI Official Symbol:
CCL23
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P55773
Protein GI Number:
308153623
Purification:
This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.
Research Area:
Immunology
Swissprot #:
P55773
User NOte:
Centrifuge vial prior to opening.