RANTES Antibody
| Code | Size | Price |
|---|
| PSI-XP-5735-M-0.5mg | 0.5mg | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: RANTES antibody was derived form BALB/c mice and belongs to the IgG2b isotype.
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
RANTES lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4
Documents
Further Information
Additional Names:
Regulated upon activation normally T expressed and presumably secreted, CCL5, TCP228, SISd, D17S136E, SCYA5, C-C motif chemokine 5, EoCP, eoCP, RANTES, SIS-delta
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of h-RANTES (100ng/mL), a concentration of 5-7 μg/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human RANTES when used with our biotinylated antigen affinity purified anti-human RANTES (XP-5257Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western Blot:
To detect hRANTES by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRANTES is 0.25-2.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
To yield one-half maximal inhibition [ND50] of the biological activity of h-RANTES (100ng/mL), a concentration of 5-7 μg/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human RANTES when used with our biotinylated antigen affinity purified anti-human RANTES (XP-5257Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western Blot:
To detect hRANTES by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRANTES is 0.25-2.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
Background:
RANTES is a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells. Recombinant RANTES protein induces a dose dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency virus (SIV). It is T cell and macrophage specific, induced by mitogens, and belongs to the intercrine beta (chemokine CC) family.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Monoclonal antibody against human RANTES designated as VL-1 was purified from ascites fluid on a Protein A affinity column.
NCBI Gene ID #:
6352
NCBI Official Name:
chemokine (C-C motif) ligand 5
NCBI Official Symbol:
CCL5
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P13501
Protein GI Number:
6175077
Research Area:
Chemokines & Cytokines, Functional Assays
Swissprot #:
P13501
User NOte:
Centrifuge vial prior to opening.