NANOG Antibody
| Code | Size | Price |
|---|
| PSI-45-931-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Porcine (pig)
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
NANOG, Nanog homeobox, HGNC:20857 , homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 38kDa band observed in Human Ovary lysates (calculated MW of 34.6kDa according to NP_079141.2). Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:This antibody was successfully used in: Vassena et al, Hum Mol Genet. 2012 Aug 1;21(15):3366-73. PMID: 22547223, Sancho-Martinez et al, Nat Commun. 2016 Feb 22;7:10743. PMID: 26899176, and Montserrat et al, Cell Transplant. 2012;21(5):815-25. PMID: 21944493.Immunofluorescence: Parts of a keratinocyte-derived colony of induced pluripotent stem cells are stained for Nanog (green).
This antibody was successfully used in IF on Human: Estibaliz Arellano-Viera et al. (2019) https://doi.org/10.1016/j.scr.2019.101410. This anyibody was successfully used in ICC on Human: Stem Cell Research (2019), Enzyme immunoassay: This antibody is reported to work in sandwich-type ELISA both as a capturer and as a reporter in combination with EB07693. This antibody does not work in PFA-fixed frozen embryos and ESC from Mouse.
This antibody was successfully used in IF on Human: Estibaliz Arellano-Viera et al. (2019) https://doi.org/10.1016/j.scr.2019.101410. This anyibody was successfully used in ICC on Human: Stem Cell Research (2019), Enzyme immunoassay: This antibody is reported to work in sandwich-type ELISA both as a capturer and as a reporter in combination with EB07693. This antibody does not work in PFA-fixed frozen embryos and ESC from Mouse.
Background References:
- Hyslop LA, Stojkovic M, Armstrong L, Walter T, Stojkovic P, Przyborski S, Herbert M, Murdoch A, Strachan T, Lako M. Downregulation of NANOG induces differentiation of Human Embryonic Stem Cells to Extraembryonic Lineages. Stem Cells. 2005 Jun 27;
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig
Immunogen:
The immunogen for this antibody is: C-QNQRMKSKRWQKNN
NCBI Gene ID #:
79923
NCBI Official Name:
Nanog homeobox
NCBI Official Symbol:
NANOG
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_079141.2
Protein GI Number:
13376298
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q9H9S0