GST Monoclonal Antibody
| Code | Size | Price |
|---|
| PSI-32-166-0.1mg | 0.1mg | £344.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 6G9B9
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
GST monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
gst, b1635, JW1627, GST B1-1
Application Note:
Western Blot:Dilution 1:2000 - 5,000
ELISA:Proposed dilution 1:10,000.
Determining optimal working dilutions by titration test.
ELISA:Proposed dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coliare in common usage; such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. coli.
Background References:
- Singlestep purification of polypeptides expressed in Escherichia colias fusions with glutathione Stransferase. 1988. Gene 67: 31-40.
Buffer:
Purified antibody in PBS containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified recombinant GST expressed in E. Coli strain M15.
NCBI Gene ID #:
12931264
NCBI Official Name:
glutathionine S-transferase
NCBI Official Symbol:
gst
NCBI Organism:
Escherichia coli str. K-12 substr. W3110
Protein Accession #:
P0A9D2
Protein GI Number:
71159393
Purification:
Antibody is purified by protein A affinity chromatography.
Swissprot #:
P0A9D2
User NOte:
Optimal dilutions for each application to be determined by the researcher.