Nucleophosmin Antibody
| Code | Size | Price |
|---|
| PSI-45-099-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
NPM1, NPM, B23, nucleophosmin (nucleolar phosphoprotein B23, numatrin), numatrin, nucleolar phosphoprotein B23, nucleophosmin/nucleoplasmin family, member 1, MGC104254, nucleophosmin 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 37kDa band observed in lysates of cell lines Daudi, Jurkat, K562 and NIH3T3 and in Mouse Spleen lysates (calculated MW of 32.6kDa according to Human NP_001341935.1 and Mouse NP_032748.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.01-1?g/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Testis. Recommended concentration: 5?g/ml. Immunofluorescence: Strong expression of the protein seen in the nucleoli of HeLa cells. Recommended concentration: 10?g/ml.
Background References:
- Grisendi S, Pandolfi PP. NPM mutations in acute myelogenous leukemia. N Engl J Med. 2005 Jan 20;352(3):291-2.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-QEAIQDLWQWRKSL
NCBI Gene ID #:
4869
NCBI Official Name:
nucleophosmin (nucleolar phosphoprotein B23, numatrin)
NCBI Official Symbol:
NPM1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001341935.1, NP_954654.1, NP_001341936.1, NP_001341939.1
Protein GI Number:
10835063
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
.
Swissprot #:
P06748