SCF Antibody

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ProSci
Product Code: PSI-XP-5265
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XP-5265-0.1mg0.1mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
SCF antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-th

Images

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To detect hSCF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human SCF (XP-5265Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hSCF.
2 / 4
To detect hSCF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSCF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
3 / 4
To detect hSCF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSCF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
4 / 4
This antibody stained CACO-2 cells. The primary antibody was incubated at 2.0 ug/ml overnight at 4˚C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Cell Profiling group, SciLifeLab Stockholm.
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To detect hSCF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human SCF (XP-5265Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hSCF.
To detect hSCF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSCF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hSCF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSCF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained CACO-2 cells. The primary antibody was incubated at 2.0 ug/ml overnight at 4˚C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Cell Profiling group, SciLifeLab Stockholm.

Documents

Further Information

Additional Names:
SF, MGF, SCF, FPH2, KL-1, Kitl, SHEP7, Kit ligand, Mast cell growth factor
Application Note:
ELISA:

To detect hSCF by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hSCF.

Sandwich:

To detect hSCF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human SCF (XP-5265Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hSCF.

Western Blot:
To detect hSCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hSCF (human SCF).
NCBI Gene ID #:
4254
NCBI Official Name:
KIT ligand
NCBI Official Symbol:
KITLG
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P21583
Protein GI Number:
134289
Purification:
Anti-hSCF specific antibody was purified by affinity chromatography employing immobilized hSCF matrix.
Research Area:
Signal Transduction,Immunology,Antibody Pairs
Swissprot #:
P21583
User NOte:
Centrifuge vial prior to opening.