IGF-II Antibody
| Code | Size | Price |
|---|
| PSI-XP-5160-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
IGF-II antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze
Documents
Further Information
Additional Names:
IGF-II, PP9974, C11orf43Insulin-like growth factor II, Somatomedin-A, IGF-II
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIGF-II (10 ng/mL), a concentration of 3.1 4.6 mg/mL of this antibody is required.
ELISA:
To detect hIGF-II by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIGF-II.
Sandwich:
To detect hIGF-II by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IGF-II (XP-5160Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-II.
Western Blot:
To detect hIGF-II by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-II is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To yield one-half maximal inhibition [ND50] of the biological activity of hIGF-II (10 ng/mL), a concentration of 3.1 4.6 mg/mL of this antibody is required.
ELISA:
To detect hIGF-II by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIGF-II.
Sandwich:
To detect hIGF-II by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IGF-II (XP-5160Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-II.
Western Blot:
To detect hIGF-II by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-II is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIGF-II (human Insulin Like Growth Factor-II).
NCBI Gene ID #:
3481
NCBI Official Name:
insulin-like growth factor 2 (somatomedin A)
NCBI Official Symbol:
IGF2
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P01344
Protein GI Number:
124255
Purification:
Anti-hIGF-II specific antibody was purified by affinity chromatography employing immobilized hIGF-II matrix.
Research Area:
Growth Factor,Antibody Pairs, Functional Assays
Swissprot #:
P01344
User NOte:
Centrifuge vial prior to opening.