MIG Antibody
| Code | Size | Price |
|---|
| PSI-XP-5231-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
MIG antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-th
Documents
Further Information
Additional Names:
CMK, MIG, Humig, SCYB9, crg-10, CMK, C-X-C motif chemokine 9, Gamma-interferon-induced monokine, HuMIG
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hMIG (100.00 ng/mL), a concentration of 5.0 - 7.0 μg/mL of this antibody is required.
ELISA:
To detect hMIG by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIG.
Sandwich:
To detect hMIG by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human MIG (XP-5231Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIG.
Western Blot:
To detect hMIG by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIG is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To yield one-half maximal inhibition [ND50] of the biological activity of hMIG (100.00 ng/mL), a concentration of 5.0 - 7.0 μg/mL of this antibody is required.
ELISA:
To detect hMIG by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIG.
Sandwich:
To detect hMIG by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human MIG (XP-5231Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIG.
Western Blot:
To detect hMIG by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIG is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
MIG, a member of the alpha-chemokine family (CXC) of cytokines, is produced by stimulated monocytes, macrophages and endothelial cells. It signals through the CXCR3 receptor.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIG (human MIG).
NCBI Gene ID #:
4283
NCBI Official Name:
chemokine (C-X-C motif) ligand 9
NCBI Official Symbol:
CXCL9
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
Q07325
Protein GI Number:
585487
Purification:
Anti-hMIG specific antibody was purified by affinity chromatography employing immobilized hMIG matrix.
Research Area:
Chemokines & Cytokines,Signal Transduction,Homeostasis,Antibody Pairs, Functional Assays
Swissprot #:
Q07325
User NOte:
Centrifuge vial prior to opening.