RANTES Antibody
| Code | Size | Price |
|---|
| PSI-XP-5257-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
RANTES antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze
Documents
Further Information
Additional Names:
SISd, eoCP, SCYA5, RANTES, TCP228, D17S136E, SIS-delta, C-C motif chemokine 5, EoCP
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hRANTES (100 ng/mL), a concentration of 5.0 - 7.0 μg/mL of this antibody is required.
ELISA:
To detect hRANTES by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hRANTES.
Sandwich:
To detect hRANTES by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human RANTES (XP-5257Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hRANTES.
Western Blot:
To detect hRANTES by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRANTES is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To yield one-half maximal inhibition [ND50] of the biological activity of hRANTES (100 ng/mL), a concentration of 5.0 - 7.0 μg/mL of this antibody is required.
ELISA:
To detect hRANTES by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hRANTES.
Sandwich:
To detect hRANTES by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human RANTES (XP-5257Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hRANTES.
Western Blot:
To detect hRANTES by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRANTES is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
RANTES is a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells. Recombinant RANTES protein induces a dose dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency virus (SIV). It is T cell and macrophage specific, induced by mitogens, and belongs to the intercrine beta (chemokine CC) family.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hRANTES (human RANTES).
NCBI Gene ID #:
6352
NCBI Official Name:
chemokine (C-C motif) ligand 5
NCBI Official Symbol:
CCL5
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P13501
Protein GI Number:
6175077
Purification:
Anti-hRANTES specific antibody was purified by affinity chromatography employing immobilized hRANTES matrix.
Research Area:
Chemokines & Cytokines,Antibody Pairs, Functional Assays
Swissprot #:
P13501
User NOte:
Centrifuge vial prior to opening.