Pyruvate Dehydrogenase Antibody

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ProSci
Product Code: PSI-45-107
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-107-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-107 (1ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-107 (1ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
PDHX, pyruvate dehydrogenase complex, component X, E3BP, OPDX, PDX1, proX, DLDBP, E3-binding protein, pyruvate dehydrogenase complex, lipoyl-containing component X, dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex, pyruvate dehydrogenase complex, E3-binding protein subunit, pyruvate dehydrogenase protein X component, mitochondrial
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa band observed in Human Pancreas lysate (calculated MW of 54.2kDa according to NP_003468.1). In transfected HEK293 transiently expressing PDHX a band of approx. 50kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-1ug/ml.
Background References:
  • Li Y, Cao X, Li LX, Brubaker PL, Edlund H, Drucker DJ. {beta}-Cell Pdx1 Expression Is Essential for the Glucoregulatory, Proliferative, and Cytoprotective Actions of Glucagon-Like Peptide-1. Diabetes. 2005 Feb;54(2):482-91.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-KSFKANLENPIRLA
NCBI Gene ID #:
8050
NCBI Official Name:
pyruvate dehydrogenase complex, component X
NCBI Official Symbol:
PDHX
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003468.2, NP_001128496.1, NP_001159630.1
Protein GI Number:
4505699
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis ,Stem Cell
SPECIFICITY:
This antibody is expected to recognise all reported isoforms.
Swissprot #:
O00330