RBP1 Antibody
Code | Size | Price |
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PSI-45-136-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
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Further Information
Additional Names:
RBP1, CRBP, RBPC, CRBP1, CRABP-I, retinol binding protein 1, cellular, retinol-binding protein 1, cellular, CRBPI
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 16kDa band observed in NIH3T3 and approx 17kDa in U251 cell lysates. The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. (calculated MW of 22.3kDa according to Human NP_002890.2 and 15.8kDa according to Mouse NP_035384.1). Recommended concentration: 0.5-3ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Expression of the protein seen in the cytoplasm and nucleus of U2OS cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Background References:
- Farias EF, Ong DE, Ghyselinck NB, Nakajo S, Kuppumbatti YS, Mira y Lopez R. Cellular retinol-binding protein I, a regulator of breast epithelial retinoic acid receptor activity, cell differentiation, and tumorigenicity. J Natl Cancer Inst. 2005 Jan 5;97(1):21-9.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: VEGVVCKQVFKKVQ
NCBI Gene ID #:
5947
NCBI Official Name:
retinol binding protein 1, cellular
NCBI Official Symbol:
RBP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002890.2
Protein GI Number:
4506451
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
.
Swissprot #:
P09455