SALL4 Antibody
Code | Size | Price |
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PSI-46-333-0.1mg | 0.1mg | £626.00 |
Quantity:
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Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
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Further Information
Additional Names:
SALL4, sal-like 4 (Drosophila) , AA407717, AL022809, AW536104, C330011P20Rik, C78083, C78563, Tex20, sal-like 4
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70kDa band observed in Rat Spleen and Testis lysates (calculated MW of 66.2kDa according to NP_958797.2). Recommended concentration: 0.1-0.3ug/ml.
Additional bands of unknown identity was also consistently observed at 28+24kDa. These bands were successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
- Wang J, Rao S, Chu J, Shen X, Levasseur DN, Theunissen TW, Orkin SH. A protein interaction network for pluripotency of embryonic stem cells. Nature. 2006 Nov 16;444(7117):364-8. Epub 2006 Nov 8.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: C-DQSKKGKGKPQN
NCBI Gene ID #:
99377 (mouse);
NCBI Official Name:
sal-like 4 (Drosophila)
NCBI Official Symbol:
Sall4
NCBI Organism:
Mus musculus
Physical State:
Liquid
Protein Accession #:
NP_780512.2, NP_958797.2, NP_958798.2
Protein GI Number:
117553631
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer ,Stem Cell
SPECIFICITY:
This antibody is expected to recognize isoform a (NP_780512.2) and isoform b (NP_958797.2).
Swissprot #:
Q8BX22