Sec61 alpha Antibody

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ProSci
Product Code: PSI-45-141
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-141-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-141 (0.3ug/ml) staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-141 (0.3ug/ml) staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
SEC61A1, HSEC61, SEC61, SEC61A, protein transport protein SEC61 alpha subunit isoform 1, sec61 homolog, Sec61 alpha 1 subunit (S. cerevisiae), Sec61 alpha 1 subunit, Sec61 alpha-1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 48kDa band observed in Human Brain (Cerebelum) lysates (calculated MW of 52.3kDa according to NP_037468.1). Recommended concentration: 0.3-1ug/ml.
Background References:
  • Gorlich D, Prehn S, Hartmann E, Kalies KU, Rapoport TA. A mammalian homolog of SEC61p and SECYp is associated with ribosomes and nascent polypeptides during translocation. Cell 1992 Oct 30;71(3):489-503
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow, Pig
Immunogen:
The immunogen for this antibody is: C-KEQSEVGSMGALLF
NCBI Gene ID #:
29927
NCBI Official Name:
Sec61 alpha 1 subunit (S. cerevisiae)
NCBI Official Symbol:
SEC61A1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_037468.1
Protein GI Number:
7019415
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
.
Swissprot #:
P61619