SHP-1 Antibody

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ProSci
Product Code: PSI-45-128
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-128-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-128 (0.2ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-128 (0.2ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
PTPN6, SHP1 , protein tyrosine phosphatase, non-receptor type 6 , HCP, HCPH, HPTP1C, PTP-1C, SH-PTP1, SHP-1, SHP-1L, 70 kda SHP-1L protein, hematopoietic cell phosphatase, hematopoietic cell protein-tyrosine phosphatase, protein-tyrosine phosphatase 1C
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in lysates of cell lines Jurkat and U937 (calculated MW of 67.7kDa according to NP_536858.1 and 67.6kDa according to NP_002822.2). Recommended concentration: 0.2-0.5ug/ml.
Background References:
  • Dubois MJ, Bergeron S, Kim HJ, Dombrowski L, Perreault M, Fournes B, Faure R, Olivier M, Beauchemin N, Shulman GI, Siminovitch KA, Kim JK, Marette A. The SHP-1 protein tyrosine phosphatase negatively modulates glucose homeostasis. Nat Med. 2006 May;12(5):549-56. Epub 2006 Apr 16.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-HTKNKREEKVKKQ
NCBI Gene ID #:
5777
NCBI Official Name:
protein tyrosine phosphatase, non-receptor type 6
NCBI Official Symbol:
PTPN6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_536858.1, NP_002822.2
Protein GI Number:
18104991
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction ,Chemokines & Cytokines
SPECIFICITY:
This antibody is expected to recognise both reported isoforms (NP_536858.1and NP_002822.2)
Swissprot #:
P29350