BAFF Antibody

Contact us
ProSci
Product Code: PSI-XP-5728-M
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XP-5728-M-0.5mg0.5mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
BAFF lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4&#

Images

1 / 3
To a sandwich ELISA (assuming 100ul/well), a concentration of 5.0-6.0 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant human BAFF when used with ProSci's biotinylated antigen affinity purified anti-human BAFF (XP-5105Bt) as the detection antibody at a concentration of approximately 0.25-0.50 ug/ml.
2 / 3
To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 2.0-4.0 ng/lane, under non-reducing conditions.
3 / 3
To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 2.0-4.0 ng/lane, under non-reducing conditions.
❮❯

To a sandwich ELISA (assuming 100ul/well), a concentration of 5.0-6.0 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant human BAFF when used with ProSci's biotinylated antigen affinity purified anti-human BAFF (XP-5105Bt) as the detection antibody at a concentration of approximately 0.25-0.50 ug/ml.
To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 2.0-4.0 ng/lane, under non-reducing conditions.
To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 2.0-4.0 ng/lane, under non-reducing conditions.

Documents

Further Information

Additional Names:
B cell Activating Factor , BAFF, BLYS, TALL1, TNFSF20, ZTNF4, UNQ401/PRO738, Tumor necrosis factor ligand superfamily member 13B, B lymphocyte stimulator, BLyS, DTL, CD257, THANK, TALL-1
Application Note:
ELISA:

In a sandwich ELISA (assuming 100 μL/well), a concentration of 5.0-6.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human BAFF when used with our biotinylated antigen affinity purified anti-human BAFF (XP-5105Bt) as the detection antibody at a concentration of approximately 0.25-0.50 μg/mL.

Western Blot:

To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 2.0-4.0 ng/lane, under non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
The monoclonal antibody was produced in BALB/c mice using recombinant human BAFF as the immunizing antigen.
NCBI Gene ID #:
10673
NCBI Official Name:
tumor necrosis factor (ligand) superfamily, member 13b
NCBI Official Symbol:
TNFSF13B
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
Q9Y275
Protein GI Number:
13124573
Purification:
This IgG1/K antibody was purified from ascites fluid on a Protein A chromatography column.
Research Area:
Chemokines & Cytokines,Cancer
Swissprot #:
Q9Y275
User NOte:
Centrifuge vial prior to opening.