LEC Antibody
| Code | Size | Price |
|---|
| PSI-XP-5722-M-0.5mg | 0.5mg | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
LEC lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4
Documents
Further Information
Additional Names:
CCL16, CKb12, HCC4, ILINCK, LCC1, Liver expressed chemokine, LMC, Monotactin 1, Mtn1, NCC4, SCYA16, SCYL4, Small inducible cytokine A16 precursor, Small inducible cytokine subfamily A (Cys Cys) member 16, C-C motif chemokine 16, Chemokine CC-4, HCC-4, LEC, LCC-1, Mtn-1, NCC-4
Application Note:
ELISA:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human LEC when used with our biotinylated antigen affinity purified anti-human LEC (XP-5722-M) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western Blot:
To detect hLEC by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLEC is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.
In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human LEC when used with our biotinylated antigen affinity purified anti-human LEC (XP-5722-M) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western Blot:
To detect hLEC by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLEC is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.
Background:
LEC displays chemotactic activity for lymphocytes and monocytes but not for neutrophils. It also shows a potent myelosuppressive activity and suppresses proliferation of myeloid progenitor cells. Its expression is upregulated by IL10.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant hLEC as the immunizing antigen.
NCBI Gene ID #:
6360
NCBI Official Name:
chemokine (C-C motif) ligand 16
NCBI Official Symbol:
CCL16
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
O15467
Protein GI Number:
6094382
Purification:
This IgG1K antibody was purified from ascites fluid on a Protein A affinity column.
Research Area:
Chemokines & Cytokines,Signal Transduction
Swissprot #:
O15467
User NOte:
Centrifuge vial prior to opening.