MIG Antibody
| Code | Size | Price |
|---|
| PSI-XP-5721-M-0.5mg | 0.5mg | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
MIG lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4
Documents
Further Information
Additional Names:
monokine induced by interferon-gamma, CMK, MIG, SCYB9, C-X-C motif chemokine 9, Gamma-interferon-induced monokine, HuMIG, Humig, crg-10
Application Note:
ELISA:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human MIG when used with our biotinylated antigen affinity purified anti-human MIG (XP-5231Bt) as the detection antibody at a concentration of approximately 1.0-2.0 μg/mL.
Sandwich:
To detect hMIG by Western Blot analysis this antibody can be used at a concentration of 0.50-2.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIG is 0.25-0.50 ng/lane, under non-reducing conditions and 2.0-4.0 ng/lane, under reducing conditions.
In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human MIG when used with our biotinylated antigen affinity purified anti-human MIG (XP-5231Bt) as the detection antibody at a concentration of approximately 1.0-2.0 μg/mL.
Sandwich:
To detect hMIG by Western Blot analysis this antibody can be used at a concentration of 0.50-2.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIG is 0.25-0.50 ng/lane, under non-reducing conditions and 2.0-4.0 ng/lane, under reducing conditions.
Background:
MIG, a member of the alpha-chemokine family (CXC) of cytokines, is produced by stimulated monocytes, macrophages and endothelial cells. It signals through the CXCR3 receptor.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
The monoclonal antibody was produced in Balb/c X ICR F1 mice using recombinant hMIG as the immunizing antigen.
NCBI Gene ID #:
4283
NCBI Official Name:
chemokine (C-X-C motif) ligand 9
NCBI Official Symbol:
CXCL9
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
Q07325
Protein GI Number:
585487
Purification:
This IgG1K antibody was purified from ascites fluid on a Protein A affinity column.
Research Area:
Chemokines & Cytokines,Signal Transduction,Homeostasis
Swissprot #:
Q07325
User NOte:
Centrifuge vial prior to opening.