MIP-1a Antibody
| Code | Size | Price |
|---|
| PSI-XP-5734-M-0.5mg | 0.5mg | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
MIP-1α lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks a
Documents
Further Information
Additional Names:
Macrophage inflammatory protein 1 alpha, CCL 3, MIP 1 alpha, MIP1a, SCYA 3, Heparin binding chemotaxis protein, G0S19-1, MIP1A, SCYA3, C-C motif chemokine 3, Macrophage inflammatory protein 1-alpha, MIP-1-alpha,
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-1α (100 ng/mL), a concentration of 5.0-10.0 μg/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 2000 pg/mL of recombinant human MIP-1α when used with our biotinylated antigen affinity purified anti-human MIP-1α (XP-5232Bt) as the detection antibody at a concentration of approximately 1.0-2.0 μg/mL.
Western Blot:
To detect hMIP-1α by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-1α is 1.0-2.0 ng/lane, under non-reducing conditions.
To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-1α (100 ng/mL), a concentration of 5.0-10.0 μg/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 2000 pg/mL of recombinant human MIP-1α when used with our biotinylated antigen affinity purified anti-human MIP-1α (XP-5232Bt) as the detection antibody at a concentration of approximately 1.0-2.0 μg/mL.
Western Blot:
To detect hMIP-1α by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-1α is 1.0-2.0 ng/lane, under non-reducing conditions.
Background:
Macrophage Inflammatory Protein 1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
The monoclonal antibody was produced in C3H/HeJ mice using recombinant hMIP-1α as the immunizing antigen.
NCBI Gene ID #:
6348
NCBI Official Name:
chemokine (C-C motif) ligand 3
NCBI Official Symbol:
CCL3
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P10147
Protein GI Number:
127078
Purification:
This IgG1 antibody was purified from ascites fluid by SAS precipitation followed by ion exchange column.
Research Area:
Immunology,Chemokines & Cytokines, Functional Assays
Swissprot #:
P10147
User NOte:
Centrifuge vial prior to opening.