DGKE Antibody

ProSci
Product Code: PSI-26-054
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-26-054-100ul100ul£662.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store DGKE antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in WB on Human brain at 0.2-1 ug/ml.
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Antibody used in WB on Human brain at 0.2-1 ug/ml.

Further Information

Additional Names:
DGKE, DAGK6, DGK, DAGK5, NPHS7
Application Note:
DGKE antibody can be used for detection of DGKE by ELISA at 1:1562500. DGKE antibody can be used for detection of DGKE by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis.Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis.
Background References:
  • Epand, R.M., (2007) Biochemistry 46 (49), 14225-14231.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DGKE.
NCBI Gene ID #:
8526
NCBI Official Name:
diacylglycerol kinase, epsilon 64kDa
NCBI Official Symbol:
DGKE
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
64 kDa
Protein Accession #:
NP_003638
Protein GI Number:
4503313
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Membrane ,Signal Transduction ,Immunology
Swissprot #:
P52429
User NOte:
Optimal dilutions for each application to be determined by the researcher.