PAX4 Antibody

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ProSci
Product Code: PSI-42-499
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-499-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-499 (0.2ug/ml) staining of Mouse Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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42-499 (0.2ug/ml) staining of Mouse Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
PAX4, paired box 4, KPD, MGC129960, MODY9, paired box gene 4, paired domain gene 4
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 37kDa band observed in Mouse Pancreas lysates and in lysates of cell lines A431, HeLa and K562 (calculated MW of 37.1kDa according to Human NP_006184.2 and 38.0kDa according to Mouse NP_035168.1). Recommended concentration: 0.2-0.6ug/ml.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant PAX4 captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog
Immunogen:
The immunogen for this antibody is: C-GKLATATSLPEDTVR
NCBI Gene ID #:
5078
NCBI Official Name:
paired box 4
NCBI Official Symbol:
PAX4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006184.2
Protein GI Number:
170784824
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Transcription ,Cancer
SPECIFICITY:
.
Swissprot #:
O43316