PNPLA3 Antibody

ProSci
Product Code: PSI-46-700
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-700-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-700 (1ug/ml) staining of Human Adrenal gland lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-700 (1ug/ml) staining of Human Adrenal gland lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
Pnpla3, patatin-like phospholipase domain containing 3, Adpn, adiponutrin
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 40-45kDa band observed in Human Adrenal gland lysates (calculated MW of 42.6kDa according to NP_473429.2). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.
Background References:
  • Steinberg GR, Kemp BE, Watt MJ. Adipocyte triglyceride lipase expression in human obesity. Am J Physiol Endocrinol Metab. 2007 Oct;293(4):E958-64. Epub 2007 Jul 3.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: EHDICPKVKSTN
NCBI Gene ID #:
80339
NCBI Official Name:
patatin-like phospholipase domain containing 3
NCBI Official Symbol:
PNPLA3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_473429.2
Protein GI Number:
144226245
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Obesity
SPECIFICITY:
This antibody is raised against a peptide derived from the Mouse protein sequence and this peptide differs from Human only in position 2.
Swissprot #:
Q9NST1