sCD40 Ligand Antibody
Code | Size | Price |
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PSI-XP-5263-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
sCD40 Ligand antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated
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Further Information
Additional Names:
IGM, IMD3, TRAP, gp39, CD154, CD40L, HIGM1, T-BAM, TNFSF5, hCD40L, CD40 ligand, T-cell antigen Gp39, CD40-L
Application Note:
ELISA:
To detect hsCD40L by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hsCD40L.
Sandwich:
To detect hsCD40-Ligand by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human sCD40-Ligand (XP-5263Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsCD40-Ligand.
Western Blot:
To detect hsCD40L by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsCD40L is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hsCD40L by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hsCD40L.
Sandwich:
To detect hsCD40-Ligand by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human sCD40-Ligand (XP-5263Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsCD40-Ligand.
Western Blot:
To detect hsCD40L by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsCD40L is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hsCD40L (human sCD40 Ligand).
NCBI Gene ID #:
959
NCBI Official Name:
CD40 ligand
NCBI Official Symbol:
CD40LG
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P29965
Protein GI Number:
231718
Purification:
Anti-hsCD40L specific antibody was purified by affinity chromatography employing immobilized hsCD40L matrix.
Research Area:
Signal Transduction,Antibody Pairs
Swissprot #:
P29965
User NOte:
Centrifuge vial prior to opening.