STAT3 Antibody

Contact us
ProSci
Product Code: PSI-45-154
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-154-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 1
45-154 (4ug/ml) staining of paraffin embedded Mouse Thymus. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
❮❯

45-154 (4ug/ml) staining of paraffin embedded Mouse Thymus. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Documents

Further Information

Additional Names:
STAT3, signal transducer and activator of transcription 3 (acute-phase response factor), APRF, DNA-binding protein APRF, acute-phase response factor, FLJ20882, MGC16063, HIES
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Immunohistochemistry:In paraffin embedded Mouse Thymus shows preferential staining of the Medulla over the Cortex. Recommended concentration: 4-6ug/ml.Immunofluorescence:Strong expression of the protein seen in the cytoplasm of HeLa and A431 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
Background References:
  • Bromberg JF, Wrzeszczynska MH, Devgan G, Zhao Y, Pestell RG, Albanese C, Darnell JE Jr. Stat3 as an oncogene. Cell. 1999 Aug 6;98(3):295-303. Erratum in: Cell 1999 Oct 15;99(2):239.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: DMELTSECATSPM
NCBI Gene ID #:
6774
NCBI Official Name:
signal transducer and activator of transcription 3 (acute-phase response factor)
NCBI Official Symbol:
STAT3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003141.2, NP_644805.1
Protein GI Number:
21618338
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
This antibody is expected to recognise isoforms 1 and 2 (as represented by NP_644805.1 and NP_003141.2 respectively)
Swissprot #:
P40763