IDH1 Antibody

ProSci
Product Code: PSI-42-395
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-395-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Yeast
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 1
42-395 (0.5ug/ml) staining of S. cerevisiae S288c lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. The blocking buffer contained non-animal derived protein. Data kindly provided by Dr. F Reggiori, Univer

42-395 (0.5ug/ml) staining of S. cerevisiae S288c lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. The blocking buffer contained non-animal derived protein. Data kindly provided by Dr. F Reggiori, Univer

Further Information

Additional Names:
IDH1, Idh1p, YNL037C, Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 38kDa band observed in wildtype lysates of S.cerevisiae (calculated MW of 39.3kDa according to NP_014361.1). Recommended concentration: 0.3-1ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-EPGSRHVGLDIKGQN
NCBI Gene ID #:
855691
NCBI Official Name:
isocitrate dehydrogenase (NAD(+)) IDH1
NCBI Official Symbol:
IDH1
NCBI Organism:
Saccharomyces cerevisiae S288c
Physical State:
Liquid
Protein Accession #:
NP_014361.1
Protein GI Number:
6324291
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
.
Swissprot #:
P28834