4E-BP1 Antibody
| Code | Size | Price |
|---|
| PSI-79-205-0.1mg | 0.1mg | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.
Documents
Further Information
Additional Names:
BP-1, 4EBP1, 4E-BP1, PHAS-I, STK14A, EIF4EBP1, PHAS-1
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Background References:
- Gingras AC, et al. (1998) Genes Dev 12 (4): 502-513. Brugarolas J, et al. (2004) Genes Dev 18 (23): 2893-2904. Kumar V, et al. (2000) EMBO J 19 (5): 1087-1097. Moody CA, et al. (2005) J Virol 79 (9): 5499-5506. Burnett PE, et al. (1998) Proc Natl Acad Sci U S A 95 (4): 1432-1437.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
4E-BP1 (Phospho-Thr45) antibody was raised against a peptide sequence around phosphorylation site of threonine 45 (S-T-T (p) -P-G) derived from Human 4E-BP1.
Modifications:
Phospho-Specific
NCBI Gene ID #:
1978
NCBI Official Name:
eukaryotic translation initiation factor 4E binding protein 1
NCBI Official Symbol:
EIF4EBP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
18 kDa
Protein Accession #:
NP_004086.1
Protein GI Number:
4758258
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific
SPECIFICITY:
This antibody detects endogenous level of 4E-BP1 only when phosphorylated at Threonine 45.
Swissprot #:
Q13541