p27Kip1 Antibody

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ProSci
Product Code: PSI-79-196
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-196-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from HeLa cells untreated or treated with EGF, IFN-α using p27Kip1 (Phospho-Thr187).
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p27Kip1 (Phospho-Thr187).
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Western blot analysis of lysed extracts from HeLa cells untreated or treated with EGF, IFN-α using p27Kip1 (Phospho-Thr187).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p27Kip1 (Phospho-Thr187).

Documents

Further Information

Additional Names:
KIP1, MEN4, CDKN4, MEN1B, P27KIP1, MAPTL, MTBT1, TAU, CDKN1B, CDN1B, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, KIP1
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Positive regulator of cyclin D-dependent kinases such as CDK4. Regulated by phosphorylation and degradation events.
Background References:
  • Eguchi H, et al. (2003) Cancer Res; 63 (15): 4739-46Le XF, et al. (2003) J Biol Chem;Connor MK, et al. (2003) ) Mol Biol Cell; 14 (1): 201-13Fujita N, et al. (2002) J Biol Chem; 277 (32): 28706-13Ishida N, et al. (2000) J Biol Chem; 275 (33): 25146-54
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
p27Kip1 (Phospho-Thr187) antibody was raised against a peptide sequence around phosphorylation site of threonine 187 (E-Q-T (p) -P-K) derived from Human p27Kip1.
Modifications:
Phospho-Specific
NCBI Gene ID #:
1027
NCBI Official Name:
cyclin-dependent kinase inhibitor 1B (p27, Kip1)
NCBI Official Symbol:
CDKN1B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
27 kDa
Protein Accession #:
NP_004055.1
Protein GI Number:
4757962
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific,Cell Cycle
SPECIFICITY:
This antibody detects endogenous level of p27Kip1 only when phosphorylated at threonine 187.
Swissprot #:
P46527