Parkin Antibody

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ProSci
Product Code: PSI-50-247
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-50-247-0.1mL0.1mL£686.00
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Bovine (Cattle)
  • Human
Application: Western Blot (WB)
Storage:
Parkin antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.

Images

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Western blot of HEK293 cells transfected with Parkin WT (Phospho) and Parkin S101 mutant (non-phospho) showing the phospho-specific immunolabeling of the ~ 52 k parkin protein. The immunolabeling is absent in the parkin S101 mutant.
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Western blot of HEK293 cells transfected with Parkin WT (Phospho) and Parkin S101 mutant (non-phospho) showing the phospho-specific immunolabeling of the ~ 52 k parkin protein. The immunolabeling is absent in the parkin S101 mutant.

Documents

Further Information

Application Note:
The antibody has been directly tested for reactivity in Western blots with human tissue. It is anticipated that the antibody will react with bovine and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Background:
Parkin is an E3 ligase in the ubiquitin-proteasome system. Hereditary Parkinson?s disease is most commonly caused by mutations in the parkin gene and is characterized by the progressive loss of dopaminergic neurons and the presence of Lewy bodies in the substania nigra (Jenner aet al.,1992). Recent evidence suggests that phosphorylation of parkin at Ser101 may have an important regulatory role on its E3 ubiquitin ligase activity (Yamamoto et al., 2005).
Background References:
  • Jenner P, Dexter DT, Sian J, Schapira AH, Marsden CD (1992) Oxidative stress as a cause of nigral cell death in Parkinson?s disease and incidental Lewy body disease. Ann Neurol. 32 Suppl: S82-7.
  • Yamamoto A, Friedlein A, Imai Y, Takahashi R, Kahle PJ, Haass C (2005) Parkin phosphorylation and modulation of its E# ubiquitin ligase activity. J Biol chem. 280(5):3390-9
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser101 of human parkin.
Modifications:
Phospho-Specific
NCBI Gene ID #:
5071
NCBI Official Name:
parkin RBR E3 ubiquitin protein ligase
NCBI Official Symbol:
PARK2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
52
Protein Accession #:
O60260
Protein GI Number:
116242725
Purification:
Affinity Purified
Research Area:
Phospho-Specific
Swissprot #:
O60260
User NOte:
Optimal dilutions for each application to be determined by the researcher.