Alpha synuclein Antibody

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ProSci
Product Code: PSI-50-241
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-50-241-0.1mL0.1mL£686.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Bovine (Cattle)
  • Canine (dog)
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
Alpha synuclein antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.

Images

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Western blot of rat cortex lysate showing specific labeling of the ~15 k alpha synuclein protein phosphorylated at Ser129. Immunolabeling is blocked by the phosphopeptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).
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Western blot of rat cortex lysate showing specific labeling of the ~15 k alpha synuclein protein phosphorylated at Ser129. Immunolabeling is blocked by the phosphopeptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).

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Further Information

Application Note:
The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, human, mouse, and non-human primate based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Background:
Alpha-synuclein is a presynaptic neuronal protein that is thought to be involved in the formation of SNARE complexes. Most significantly, aggregated alpha-synuclein is one of the major components found in the Lewy bodies that occur in Parkinson?s disease (PD) and other neurodegenerative disorders (Okochi et al., 2000). Mutation of the alpha-synuclein gene is associated with familial forms of PD (Newmann et al., 2002). Recent evidence suggests that phosphorylation of alpha-synuclein at ser129 plays a role in the formation of inclusions in PD (Smith et al., 2005; Masliah et al., 2005).
Background References:
  • Okochi M, Walter J, Koyama A, Nakajo S, Baba M, Iwatsubo T, Meijer L, Kahle PJ, Haass C (2000) Constitutive phosphorylation of the Parkinson?s disease associated alpha-synuclein. J Biol Chem Jan 7;275(1): 390-7.
  • Neumann M, Kahle PJ, Giasson BI, Ozmen L, Borroni E, Spooren W, Muller V, Odoy S, Fujiwara H, Hasegawa M, Iwatsubo T, Trojanowski JQ, Kretzschmar HA, Haass C (2002) Misfolded proteinase k resistant hyperphosphorylated alpha-synuclein in aged transgenic mice with locomotor deterioration and in human alpha synucleinopathies. J Clin Invest Nov; 110(10):1403-5.
  • Smith WW, Margolis RL, Li X, Troncoso JC, Lee MK, Dawson VL, Dawson TM, Iwatsubo T, Ross CA (2005) Alpha-synuclein phosphorylation enhances eosinophilic cytoplasmic inclusion formation in SH-SY5Y Cells. J Neurosci June 8; 25(23):5544-5552.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser129 of alpha synuclein.
Modifications:
Phospho-Specific
NCBI Gene ID #:
29219
NCBI Official Name:
synuclein, alpha (non A4 component of amyloid precursor)
NCBI Official Symbol:
Snca
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
15
Protein Accession #:
P37377
Protein GI Number:
586065
Purification:
Affinity Purified
Research Area:
Phospho-Specific
Swissprot #:
P37377
User NOte:
Optimal dilutions for each application to be determined by the researcher.