MEK5 Antibody

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ProSci
Product Code: PSI-50-245
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-50-245-0.1mL0.1mL£686.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Amphibian
  • Avian
  • Bovine (Cattle)
  • Canine (dog)
  • Fish
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
MEK5 antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.

Images

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Western blot of rat testes lysate showing specific labeling of the ~49k MEK5 protein (Control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).
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Western blot of rat testes lysate showing specific labeling of the ~49k MEK5 protein (Control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).

Documents

Further Information

Application Note:
The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with mouse, human, chicken, bovine, canine, non-human primate, Xenopus, and Zebrafish tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Background:
MEK5 (also known as MKK5) is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family. MEK5 has been shown to specifically activate ERK5 (Zhou et al., 1995) whereas MEK5 itself is regulated by MEKK3 (Chao et al., 1999). An important link between MEK5 and metastatic prostrate cancer has been demonstrated (Mehta et.al., 2003). Dual phosphorylation of Ser311 and Thr315 have been implicated in cell proliferation (Cameron et al., 2004).
Background References:
  • Zhou G, Bao ZQ, Dixon JE, (1995) Components of a new human protein kinase signal transduction pathway. J. Biol. Chem. 270(21):12665-9.
  • Chao TH, Hayashi M, Tapping RI, Kato Y, Lee JD (1999) MEKK3 directly regulates MEK5 activity as part of the big mitogen-activated protein kinase 1 (BMK1) signaling pathway. J. Biol. Chem. 274(51):36035-8.
  • Mehta PB, Jenkins BL, McCarthy L, Thilak L, Robson CN, Neal DE, Leung HY (2003) MEK5 overexpression is associated with metastatic prostrate cancer, and stimulates proliferation, MMP-9 expression and invasion. Oncogene 22(9):1381-9.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser311, Thr315 of MEK5.
Modifications:
Phospho-Specific
NCBI Gene ID #:
29568
NCBI Official Name:
mitogen activated protein kinase kinase 5
NCBI Official Symbol:
Map2k5
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
49
Protein Accession #:
Q62862
Protein GI Number:
2499634
Purification:
Affinity Purified
Research Area:
Phospho-Specific
Swissprot #:
Q62862
User NOte:
Optimal dilutions for each application to be determined by the researcher.