Connexin 43 Antibody

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ProSci
Product Code: PSI-50-220
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-50-220-0.1mL0.1mL£686.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Avian
  • Bovine (Cattle)
  • Canine (dog)
  • Cavia (Guinea pig)
  • Fish
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
Connexin 43 antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.

Images

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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~43k connexin 43 phosphorylated at Ser368 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lamda-Ptase). The blot is identical to the control except that it was incubated in lamda-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser368 connexin 43 antibody. The immunolabeling of connexin 43 is completely eliminated by treatment with lamda-Ptase.
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~43k connexin 43 phosphorylated at Ser368 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lamda-Ptase). The blot is identical to the control except that it was incubated in lamda-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser368 connexin 43 antibody. The immunolabeling of connexin 43 is completely eliminated by treatment with lamda-Ptase.

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Further Information

Application Note:
The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, guinea pig, human, mouse and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Background:
Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy (Aronica et al., 2001). Connexin 43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of connexin 43 is known to be regulated by phosphorylation at Ser368 by protein kinase C (Yogo et al., 2002; Bao et al., 2004a). Phosphorylation of Ser368 by PKC induces a conformational change of connexin 43 that results in a decrease in gap junction permeability (Bao et al., 2004b).
Background References:
  • Aronica E, Gorter JA, Jansen GH, Leenstra S, Yankaya B, Troost D (2001) Expression of connexin 43 and connexin 32 gap-junction proteins in epilepsy-associated brain tumors and in the perilesional epileptic cortex. Acta Neuropathol (Berl) 101:449-459.
  • Bao X, Altenberg GA, Reuss L (2004a) Mechanism of regulation of the gap junction protein connexin 43 by protein kinase C-mediated phosphorylation. Am J Physiol Cell Physiol 286:C647-C654.
  • Bao X, Reuss L, Altenberg GA (2004b) Regulation of purified and reconstituted connexin 43 hemichannels by protein kinase C-mediated phosphorylation of Serine 368. J Biol Chem 279:20058-20066.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser368 of rat connexin 43.
Modifications:
Phospho-Specific
NCBI Gene ID #:
24392
NCBI Official Name:
gap junction protein, alpha 1
NCBI Official Symbol:
Gja1
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
43
Protein Accession #:
P08050
Protein GI Number:
117708
Purification:
Affinity Purified
Research Area:
Phospho-Specific
Swissprot #:
P08050
User NOte:
Optimal dilutions for each application to be determined by the researcher.