Munc-18 Antibody

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ProSci
Product Code: PSI-50-230
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-50-230-0.1mL0.1mL£686.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Amphibian
  • Avian
  • Bovine (Cattle)
  • Canine (dog)
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Munc-18 antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.

Images

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Western blot of rat cortex lysate showing specific immunolabeling of the ~65k Munc-18 protein phosphorylated at Ser515. Immunolabeling is blocked by the phosphopeptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).
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Western blot of rat cortex lysate showing specific immunolabeling of the ~65k Munc-18 protein phosphorylated at Ser515. Immunolabeling is blocked by the phosphopeptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).

Documents

Further Information

Application Note:
The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primates and Xenopus based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Background:
Munc-18 ( mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part, to it ability to bind syntaxin (Ciufo et al., 2005). Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004). The function of Munc-18 is thought to be regulated by PKC phosphorylation of Ser515 on the Munc-18 protein (Sassa et al., 1996).
Background References:
  • Ciufo LF, Barclay JW, Burgoyne RD, Morgan A (2005) Munc18-1 regulates early and late stages of exocytosis via syntaxin-independent protein interactions. Mol Biol Cell 16:470-482.
  • Liu J, Ernst SA, Gladycheva SE, Lee YY, Lentz SI, Ho CS, Li Q, Stuenkel EL (2004) Fluorescence resonance energy transfer reports properties of syntaxin1a interaction with Munc18-1 in vivo. J Biol Chem 279:55924-55936.
  • Sassa T, Ogawa H, Kimoto M, Hosono R (1996) The synaptic protein UNC-18 is phosphorylated by protein kinase C. Neurochem Int 29:543-552.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser515 of Munc-18.
Modifications:
Phospho-Specific
NCBI Gene ID #:
81804
NCBI Official Name:
syntaxin binding protein 2
NCBI Official Symbol:
Stxbp2
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
65
Protein Accession #:
Q62753
Protein GI Number:
2501516
Purification:
Affinity Purified
Research Area:
Phospho-Specific
Swissprot #:
Q62753
User NOte:
Optimal dilutions for each application to be determined by the researcher.