Raf-1 Antibody

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ProSci
Product Code: PSI-50-238
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-50-238-0.1mL0.1mL£686.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Avian
  • Bovine (Cattle)
  • Canine (dog)
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
Raf-1 antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.

Documents

Further Information

Application Note:
The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, and non human primates based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Background:
The Ras pathway is a critical signal transduction cascade involved in regulating cellular proliferation, differentiation, survival, and oncogenic transformation. Members of the Raf serine/threonine kinase family are key intermediates in this cascade, functioning to relay signals from activated Ras to the downstream protein kinases MEK and ERK (Marshall, 1996). Previous studies have shown that phosphorylation is required for Raf-1 activation (Dhillon and Kolch, 2002; Chong et al., 2003). Recent work has demonstrated that phosphorylation also regulates the downregulation of Raf (Dougherty et al., 2005) with two sites participating: Ser301 and Ser642.
Background References:
  • Chong H, Vikis HG, Guan KL (2003) Mechanisms of regulating the Raf kinase family. Cellular Signalling 15:463-469.
  • Dhillon AS, Kolch W (2002) Untying the regulation of the Raf-1 kinase. Arch Biochem Biophys 404:3-9.
  • Dougherty MK, Muller J, Ritt DA, Zhou M, Zhou XZ, Copeland TD, Conrads TP, Veenstra TD, Lu KP, Morrison DK (2005) Regulation of Raf-1 by Direct Feedback Phosphorylation. Mol Cell 17:215-224.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser642 of rat Raf-1.
Modifications:
Phospho-Specific
NCBI Gene ID #:
24703
NCBI Official Name:
v-raf-leukemia viral oncogene 1
NCBI Official Symbol:
Raf1
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
74
Protein Accession #:
P11345
Protein GI Number:
125653
Purification:
Affinity Purified
Research Area:
Phospho-Specific
Swissprot #:
P11345
User NOte:
Optimal dilutions for each application to be determined by the researcher.