Glycogen synthase Antibody

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ProSci
Product Code: PSI-50-228
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-50-228-0.1mL0.1mL£686.00
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Amphibian
  • Avian
  • Bovine (Cattle)
  • Canine (dog)
  • Fish
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
Glycogen synthase antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.

Images

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Western blot of rat cortex lysate showing specific immunolabeling of the ~46k GSK3beta protein phosphorylated at Ser9 (control). Immunolabeling is blocked by the phospho-peptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).
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Western blot of rat cortex lysate showing specific immunolabeling of the ~46k GSK3beta protein phosphorylated at Ser9 (control). Immunolabeling is blocked by the phospho-peptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).

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Further Information

Application Note:
The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with human, mouse, chicken, bovine, canine, non-human primate, Zebra fish and Xenopus based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Background:
Glycogen synthase kinase 3 (GSK3) is a serine/threonine kinase that is involved in the regulation of many signaling pathways. To date, 2 isoforms have been identified: GSK3alpha and GSK3beta. Specifically, GSK3beta has been shown to play a key inhibitory role in both the insulin and Wnt signaling pathways (Papkoff and Aikawa 1998). It has been suggested that Ser9 phosphorylation underlies the inhibition of GSK3beta by insulin (Sutherland et al., 1993).
Background References:
  • Sutherland C, Leighton IA, Cohen P (1993) Inactivation of glycogen synthase kinase 3 beta by phosphorylation: new kinase connections in insulin and growth factor signaling. Biochem J. Nov 15; 296 (Pt. 1):15-9.
  • Papkoff J, Aikawa M (1998) WNT-1 and HGF regulate GSK3b activity and beta catenin signaling in mammary epithelial cells. Biochem Biophys Res Commun. Jun 29;247(3):851-8.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser9 of GSK3b.
Modifications:
Phospho-Specific
NCBI Gene ID #:
84027
NCBI Official Name:
glycogen synthase kinase 3 beta
NCBI Official Symbol:
Gsk3b
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
46
Protein Accession #:
P18266
Protein GI Number:
125374
Purification:
Affinity Purified
Research Area:
Phospho-Specific
Swissprot #:
P18266
User NOte:
Optimal dilutions for each application to be determined by the researcher.