PLK1 Antibody

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ProSci
Product Code: PSI-50-249
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-50-249-0.1mL0.1mL£686.00
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Amphibian
  • Bovine (Cattle)
  • Canine (dog)
  • Fish
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
PLK1 antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.

Images

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Western blot of rat synaptic membrane showing specific immunolabeling of the ~66 k PLK1protein phosphorylated at Thr210 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Thr210 PLK1 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.
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Western blot of rat synaptic membrane showing specific immunolabeling of the ~66 k PLK1protein phosphorylated at Thr210 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Thr210 PLK1 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.

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Further Information

Application Note:
The antibody has been directly tested for reactivity in Western blots in rat and human tissues. It is anticipated that the antibody will react with bovine, canine, mouse, non-human primate, Xenopus and Zebrafish tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Background:
Polo-like kinases are important regulators of cell cycle progression. PLK1 is a highly conserved Ser/Thr kinase that has essential roles in the formation of mitotic bipolar spindles (van Vugt et al., 2004). Deregulated expression of PLK?s is detected in many types of cancer and associated with oncogenesis (Takei et al., 2005). It has been proposed that PLK1 function is altered at different stages of mitosis through consecutive phosphorylation events at Ser137 and Thr210 (van de Weerdt et al., 2005).
Background References:
  • van Vugt MA, van de Weerdt BC, Vader G, Janssen H, Calafat J, Klompmaker R, Wolthuis RM, Medema RH (2004) Polo-like kinase 1 is required for bipolar spindle formation but is dispesible for anaphase promoting complex cdc20 activation and initiation of cytokinesis. J. Biol. Chem. Aug 27;279(35):36841-54.
  • Takai N, Hamanaka R, Yoshimatsu J, Miyakawa I (2005) Polo-like kinases and cancer. Oncogene Jan 10; 24(2): 287-91.
  • van de Weerdt BC, van Vugt MA, Lindon C, Kauw JJ, Rozendaal MJ, Klompmaker R, Wolthuis RM, Medema RH (2005) Uncoupling anaphase-promoting complex/cyclosome activity from spindle assembly checkpoint control by deregulating polo-like kinase 1. Mol. Cell Biol. Mar; 25(5):2031-44.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr210 of PLK1.
Modifications:
Phospho-Specific
NCBI Gene ID #:
5347
NCBI Official Name:
polo-like kinase 1
NCBI Official Symbol:
PLK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
66
Protein Accession #:
P53350
Protein GI Number:
1709658
Purification:
Affinity Purified
Research Area:
Phospho-Specific
Swissprot #:
P53350
User NOte:
Optimal dilutions for each application to be determined by the researcher.