GABAB Receptor Antibody

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ProSci
Product Code: PSI-50-226
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-50-226-0.1mL0.1mL£686.00
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Amphibian
  • Avian
  • Bovine (Cattle)
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
GABAB Receptor antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.

Images

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Western blot of rat synaptic membrane showing specific immunolabeling of the ~102 k GABAB R2 protein phosphorylated at Ser783 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lamda-Ptase). The blot is identical to the control except that it was incubated in lamda-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser783 GABAB antibody. The immunolabeling is completely eliminated by treatment with lamda-Ptase.
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Western blot of rat synaptic membrane showing specific immunolabeling of the ~102 k GABAB R2 protein phosphorylated at Ser783 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lamda-Ptase). The blot is identical to the control except that it was incubated in lamda-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser783 GABAB antibody. The immunolabeling is completely eliminated by treatment with lamda-Ptase.

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Further Information

Application Note:
The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with mouse, human, non-human primate, chicken, bovine and Xenopus tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Background:
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAB receptors are heterodimeric G protein-coupled receptors that mediate slow synaptic inhibition in the central nervous system. It has recently been demonstrated that AMPK binds directly to GABAB receptors and phosphorylates S783 in the cytoplasmic tail of the R2 subunit and that S783 plays a critical role in enhancing neuronal survival after ischemia as phosphorylation of S783 is evident in many brain regions and is increased dramatically after ischemic injury to the brain (Kuramoto et al., 2007).
Background References:
  • Kuramoto N, Wilkins ME, Fairfax BP, Revilla-Sanchez R, Terunuma M, Tamaki K, Iemata M, Warren N, Couve A, Calver A, Horvath Z, Freeman K, Carling D, Huang L, Gonzales C, Cooper E, Smart TG, Pangalos MN, Moss SJ (2007) Phospho-dependent functional modulation of GABA(B) receptors by the metabolic sensor AMP-dependent protein kinase. Neuron 53:233-247.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser783 of GABAB R2.
Modifications:
Phospho-Specific
NCBI Gene ID #:
83633
NCBI Official Name:
gamma-aminobutyric acid (GABA) B receptor 2
NCBI Official Symbol:
Gabbr2
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
102
Protein Accession #:
O88871
Protein GI Number:
12643481
Purification:
Affinity Purified
Research Area:
Phospho-Specific ,Neuroscience
Swissprot #:
O88871
User NOte:
Optimal dilutions for each application to be determined by the researcher.