GABAB Receptor Antibody

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ProSci
Product Code: PSI-50-227
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-50-227-0.1mL0.1mL£686.00
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Bovine (Cattle)
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
GABAB Receptor antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.

Images

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Western blot of rat synaptic membrane showing specific immunolabeling of the ~102k GABAB R1 protein phosphorylated at Ser923 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lamda-Ptase). The blot is identical to the control except that it was incubated in lamda-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser923 GABAB antibody. The immunolabeling is completely eliminated by treatment with lamda-Ptase.
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Western blot of rat synaptic membrane showing specific immunolabeling of the ~102k GABAB R1 protein phosphorylated at Ser923 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lamda-Ptase). The blot is identical to the control except that it was incubated in lamda-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser923 GABAB antibody. The immunolabeling is completely eliminated by treatment with lamda-Ptase.

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Further Information

Application Note:
The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, human, mouse and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Background:
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAB receptors are heterodimeric G protein-coupled receptors that mediate slow synaptic inhibition in the central nervous system. Phosphorylation enhances GABAB receptor effector coupling (Couve et al., 2004). Phosphorylation of Ser923 is thought to be important in the regulation of GABAB receptor function.
Background References:
  • Couve A, Calver AR, Fairfax B, Moss SJ, Pangalos MN (2004) Unravelling the unusual signaling proerties of the GABA(B) receptor. Biochem. Pharmacol. Oct 12;68(8):1527-36.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser923 of GABAB R1.
Modifications:
Phospho-Specific
NCBI Gene ID #:
81657
NCBI Official Name:
gamma-aminobutyric acid (GABA) B receptor 1
NCBI Official Symbol:
Gabbr1
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
102
Protein Accession #:
Q9Z0U4
Protein GI Number:
12643494
Purification:
Affinity Purified
Research Area:
Phospho-Specific ,Neuroscience
Swissprot #:
Q9Z0U4
User NOte:
Optimal dilutions for each application to be determined by the researcher.