DDX25 Antibody

ProSci
Product Code: PSI-28-491
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-28-491-100ul100ul£662.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Canine (dog)
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store DDX25 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in WB on Human Jurkat 0.2-1 ug/ml.

Antibody used in WB on Human Jurkat 0.2-1 ug/ml.

Further Information

Additional Names:
DDX25, GRTH
Application Note:
DDX25 antibody can be used for detection of DDX25 by ELISA at 1:312500. DDX25 antibody can be used for detection of DDX25 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
DDX25 is a member of DEAD box proteins family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis,spermatogenesis, and cellular growth and division. DDX25 is a gonadotropin-regulated and developmentally expressed testicular RNA helicase. It may serve to maintain testicular functions related to steroidogenesis and spermatogenesis.DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The encoded protein is a gonadotropin-regulated and developmentally expressed testicular RNA helicase. It may serve to maintain testicular functions related to steroidogenesis and spermatogenesis.
Background References:
  • A, Z., et al., (2006) Hum. Reprod. 21 (3), 755-759.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DDX25.
NCBI Gene ID #:
29118
NCBI Official Name:
DEAD (Asp-Glu-Ala-Asp) box helicase 25
NCBI Official Symbol:
DDX25
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
41 kDa
Protein Accession #:
NP_037396
Protein GI Number:
164419732
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
RNA Binding
Swissprot #:
Q9UHL0-2
User NOte:
Optimal dilutions for each application to be determined by the researcher.